The film, which released on June 22, 1990, revolves around a man who wants his son to marry the daughter of a millionaire.
The 48-year-old actress took to micro-blogging website Twitter to share her excitement.
"Hum pyaar karne waale... Can't believe it's #25yearsofDil. Feels like yesterday! Loved working on this movie," Madhuri tweeted on Monday night.
She also thanked the film's director and Aamir.
She wrote: "Got me my first Filmfare award... Thanks to @aamir_khan, Indra Kumar and Baba Aazmi & Ashok Thakeria."
Anupam, who played the role of Aamir's character's father in the film, said the film is one of his favourites.
"'Dil' will always remain one of my favourote films. I loved doing the role of Hazari Prasad," he tweeted.
"Dil" was remade in Telugu in 1993 under the title "Tholi Muddhu", starring Divya Bharti and Prashanth in leading roles.