The film also won Best Director award for British director Danny Boyle and Best Writer award for Simon Beaufoy.
The 66th annual Golden Globe awards run by the Hollywood Foreign Press Association, a group of about 90 reporters covering show business for overseas outlets, were presented at a glittering ceremony in Beverly Hills, California Sunday night.
"Golden Globes, or the GGs as we very affectionately refer to them - your mad, pulsating affection for our film is much appreciated. Really, deeply appreciated, " Boyle said.
Sunday's award was a first Golden Globe for Boyle, who is best known for his cult 1996 movie "Trainspotting", "The Beach" and "28 Days Later".
His latest offering won over "The Curious Case Of Benjamin Button", "Frost/Nixon", "The Reader" and "Revolutionary Road".
"We really weren't expecting to be here in America at all at one time, so it's just amazing to be here, " said Beaufoy, whose winning script was adapted from Indian diplomat Vikas Swarup's novel "Q & A".
Bollywood super star Shah Rukh Khan was one of the presenters. Also present were "Slumdog" stars Anil Kapoor, Dev Patel, and Freida Pinto.
British Indian actor Dev Patel plays the protagonist in the film telling the heartwarming story of an 18-year-old orphan from the slums of Mumbai, who goes on to win a staggering Rs.20 million (approx $412, 000) on India's "Who Wants To Be A Millionaire?" game show.
Rahman also won the Best Composer award Friday as "Slumdog Millionaire" swept the 14th Annual Critics' Choice Awards winning five top prizes including Best Picture of 2008. The film also won Best Director for Boyle, Best Writer for Simon Beaufoy, and Best Young Actor/Actress for Dev Patel.
Besides the Golden Globes, "Slumdog Millionaire" has now won nominations for the top award by all five of the US film industry's major artistic guilds - Screen Actors Guild, Producers Guild of America, Directors Guild of America, Writers Guild of America, and American Society of Cinematographers.
Members of these groups represent a substantial block of the 5, 810 voting members of the Academy of Motion Picture Arts and Sciences which will award the Oscars Feb 22.
Meanwhile, the film took its total gross earning to over $34 million to surpass the $32.5 million gross of "Bend it Like Beckham". It has also exceeded the combined grosses of "The Namesake" ($13.6 million) and "Monsoon Wedding ($13.9 million).
With a weekend gross of $3.7 million, down 21 percent from last weekend, it scored a per-theatre average of $6, 206.
The following is a list of winners of the Golden Globe Awards in the major categories.
Best Motion Picture, Drama: "Slumdog Millionaire"
Best Motion Picture, Musical or Comedy: "Vicky Christina Barcelona"
Foreign Language Picture: "Waltz With Bashir"
Best Director: Danny Boyle, "Slumdog Millionaire"
Best Dramatic Actor: Mickey Rourke, "The Wrestler"
Best Dramatic Actress: Kate Winslet, "Revolutionary Road"
Best Actor, Comedy or Musical: Colin Farrel, "In Bruges"
Best Actress, Comedy or Musical: Sally Hawkins, "Happy-Go-Lucky"
Supporting Actor: Heath Ledger, "The Dark Knight"
Supporting Actress: Kate Winslet, "The Reader"
Screenplay: Simon Beaufoy, "Slumdog Millionaire"
Original Score: a.R. Rahman "Slumdog Millionaire"
Animated Film: "WALL-E"
Song: Bruce Springsteen, "The Wrestler"
Dramatic TV Series: "Mad Men"
Best Actor, TV Drama: Gabriel Byrne, "In Treatment"
Best Actress, TV Drama: Anna Paquin, "True Blood"
TV Series, Musical or Comedy: "30 Rock"
Best Actor, TV Musical or Comedy: Alec Baldwin, "30 Rock"
Best Actress, TV Musical or Comedy: Tina Fey, "30 Rock"
Best Mini-Series or Motion Picture made for Television: "John Adams"
Cecil B. Demille Lifetime Achievement Award: Steven Spielberg