"While most tweets are objective, frank and warm... the ones that are constantly making tactless statements about my personal life are upsetting, " Karan wrote.
"Critical appraisal and analysis is more than welcome...but insinuations and comments about my life, choices and orientation are NOT..., " he added.
Actress Sonam Kapoor tweeted in favour of Karan, suggesting he should block such people.
"Block them on your twitter account, they are haters who are just jealous, " she wrote.
Right now he is busy completing his untitled film with Kajol, Kareena Kapoor and Arjun Rampal. The movie is an adaptation of Chris Columbus' 1998 movie "Stepmom".
Karan is also expected to start shooting for the "Dostana" sequel with Abhishek Bachchan and John Abraham this summer.