With Konstas well on top of India's bowlers on day one, Kohli was booed by the Melbourne Cricket Ground after bumping into the 19-year-old as he walked down the pitch for a chat with opening partner Usman Khawaja at the end of the 10th over.
Kohli and Konstas turned to face each other and exchanged words, prompting umpire Michael Gough and Khawaja to step in to defuse the moment.
Former Australia captain Ricky Ponting, who was covering the game for host broadcaster Seven Network, said veteran batsman Kohli was clearly the offender.
"That man (Kohli) might have a few questions to answer," Ponting said.
"When you’re out there batting, the batsman owns the wicket. The crease is his. Especially between overs like that."
Former England captain Michael Vaughan also put the blame on 36-year-old Kohli.
"Virat Kohli is such an experienced pro, being wound up by a 19 (year old)," the Fox Cricket pundit said.
"It's Virat that walks into Sam."
Kohli and Konstas come together and make contact 👀#AUSvIND pic.twitter.com/adb09clEqd
— 7Cricket (@7Cricket) December 26, 2024
Konstas, who replaced the dropped Nathan McSweeney in the side, appeared unruffled by the clash, smashing Jasprit Bumrah for four on the next delivery and hitting a six over the Indian pace spearhead's head on the way to 18 runs from the over.
Konstas downplayed the incident in a brief interview with Fox Cricket during a drinks break.
"Whatever (happens) on the field stays on the field. I love competing. It doesn’t get any better for a debut at a packed stadium," he said.
It is unclear whether any action will be taken against either player.
The International Cricket Council bans "inappropriate physical contact" and those found guilty can be suspended for matches if the offence is deemed serious enough.
Batting maestro Kohli had shrugged off a dire run of form with a century in the series-opener in Perth but has managed just 21 runs in three innings since in the series.
With the temperature racing past 30 degrees Celsius in the morning, his mood may not have been helped by Konstas surviving a nick off Bumrah that fell just short of him in the slips.
Kohli had earlier laughed openly at Konstas when the teenager botched two attempts to scoop Bumrah behind the wicket.
But there was little mirth among the Indians as Konstas succeeded on his next three efforts, scoring boundaries on each.
Having also smashed fiery seamer Mohammed Siraj around the ground, Konstas was eventually dismissed for 60 off 65 balls when trapped lbw by Ravindra Jadeja before lunch.
What ICC Rulebook Says On The Incident:
The incident comes under Law 2.12: Inappropriate physical contact with a Player, Player Support Personnel, Umpire, Match Referee, or any other person (including a spectator) during an International Match.
The rule further says: "Note: Any form of inappropriate physical contact is prohibited in cricket. Without limitation, Players will breach this regulation if they deliberately, recklessly and/or negligently walk or run into or shoulder another Player or Umpire. When assessing the seriousness of the breach, the following factors (without limitation) shall be taken into account: (i) the context of the particular situation, including, without limitation, whether the contact was deliberate (i.e. intentional), reckless, negligent, and/or avoidable; (ii) the force of the contact; (iii) any resulting injury to the person with whom contact was made; and (iv) the person with whom contact was made. Level 1 (Level 1 is available only in the case of contact with Players, Player Support..."
The final decision on the matter will be taken by the ICC match referee Andy Pycroft. If Pycroft feels it was a Level 2 offence, Kohli could be handed 3-4 demerit points. Such a case could see Kohli being suspended from participating in the next match. If only a Level 1 offense is considered, Kohli could escape with just a fine.