Anderson, 34, whose barbed-wire tattoo rings her left arm, said in a statement she has been undergoing outpatient treatment for the disease at the University of California, Los Angles Medical Center.
"I contracted Hepatitis C while sharing a tattoo needle with my ex-husband Tommy Lee," she said.
"Tommy has the disease and never disclosed it to me during our marriage," said Anderson, who is embroiled in a child custody battle with Lee, the former drummer for the hard-rock band Motley Crue.
A statement issued by Lee's spokeswoman called Anderson's claims a "clear attack on Tommy" tied to their custody dispute.
Lee's statement did not directly address allegations that Anderson contracted hepatitis by sharing a tattoo needle with the musician or that he had the disease and failed to reveal it to her.
The former Playboy Playmate, who also stars in the syndicated TV series VIP, is seeking full custody of her two sons with Lee, whom she divorced in 1998 following his arrest for hitting her.
Lee pleaded no contest to a felony charge of spousal abuse for striking Anderson in the driveway of their Malibu home in February of that year and was sentenced to six months in jail and three years' probation.
In April 1999, the Canadian-born Anderson said they were attempting a reconciliation, but the couple parted ways again. Most recently, Anderson has been dating recording star Kid Rock.
Hepatitis C, the most serious form of Hepatitis, is a virus that results in inflammation, then scarring of the liver, a condition known as cirrhosis. In 20 per cent of cases, the virus ultimately leads to liver failure and death.
Up to 46 per cent of Hepatitis patients can be successfully treated with recent advances in medication administered for the disease.
