Sung by Alia, the song was posted on the video-sharing site in July 2014.
Filmmaker Karan Johar, who produced the Shashank Khaitan directorial, took to Twitter on Thursday to share the news with his fans.
"50 million for #samjhawanunplugged! You go girl @aliaa08," he tweeted.
The video of the song features Alia singing on stage while in-between various scenes from the film are shown.
"Humpty Sharma Ki Dulhania" also starred Ashutosh Rana and Siddharth Shukla, among others.