"I choose to entertain people for a living, but animals in zoos are locked in barren enclosures and put on display against their will, " she said in a statement.
"Hundreds of thousands of animals languish in zoos around the world. Because these animals are denied everything that is natural and important to them, they express their frustration and loneliness through obsessive, repetitive and even self-destructive behaviour, " she added.
This is not the first time Anushka has featured in a PETA ad. She appeared in PETA's 30-second public service announcement with her father Pt Ravi Shankar to encourage the government to increase penalty for cruelty to animals.