The 35-year-old will endorse the brand's various product range along with their latest running and fitness campaign called Move Faas, which can be seen across Puma stores in India.
"Fitness is gaining popularity in the country and Puma's 'Faastest Indian' will take it to the people in the country in their unique, uber cool style," Chitrangada said in a statement.
Rajiv Mehta, managing director, Puma South Asia, said: "Running and Fitness is an important category for us and we're really excited to have Chitrangada Singh as our brand ambassador for the range. She comes from an army background so sports was an integral part of her growing up days."