"Of all the preposterous things that have been written about Jennifer, this one takes the cake. Jennifer Aniston is not a lesbian," Kevin Huvane, a high-powered Hollywood publicist appointed by Aniston, was quoted as saying by the Internet Media Database.
The scandalous claims, which state that Aniston - the former "Friends" star - is in love with a female relative of an unnamed male movie star in New York, were posted on the website productshopnyc.com.
"Troy" star Brad Pitt and Aniston, who had been married for around four years, announced their split in January.